Biner Vector Optimation Transfer of ACC oxidase (ACO) Silencing Gene to The Plant Tissue Pisang Ambon (Musa acuminata sp. AAA) by Agrobacterium Transformation Method.
Abstract
An Agrobacterium-mediated plant transformation system was developed for the production of transgenic plant [Musa acuminata sp. AAA]. Callus were transformed using Agrobacterium tumefaciens strain GV 3850 with the binary vector pbinSRNACatN, having the kanamycin resistance gene as a selection marker. The kanamycin resistant callus were regenerated 4 to 5 weeks after co-cultivation of explants with Agrobacterium. The step selection procedure allowed the regeneration of callus which were uniformly transformed. The integration of the CaMV gene was confirmed by polymerase chain reaction (PCR). This process does incorporate steps using in vitro cell cultures or uses micropropagation, which has the important advantage that it allows regeneration of homogeneous populations of plants in a short period of time. This study shows the enormous potential for genetic manipulation of Musa species for inhibiting senescence by limiting ethylene production using RNA interference method.
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